APOPTOSIS GENE HUNTING USING RETROVIRAL EXPRESSION CLONING
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چکیده
منابع مشابه
Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector
DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and...
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ژورنال
عنوان ژورنال: TheScientificWorldJOURNAL
سال: 2001
ISSN: 1537-744X
DOI: 10.1100/tsw.2001.23.154